Oregon State University’s Steve Strauss led an international collaboration that showed the CRISPR Cas9 gene editing technique could be used with nearly 100% efficiency to knock out LEAFY, the master gene behind flower formation.
“The flowers never developed to the point where ovules, pollen or fertile seeds were observed,” Strauss said. “And there was no detectable negative effect on tree growth or form. A field study should be the next step to take a more careful look at stability of the vegetative and floral sterility traits, but with physical gene mutation we expect high reliability over the life of the trees.”
Strauss, Ph.D. student Estefania Elorriaga and research assistant Cathleen Ma teamed up with scientists at the University of Colorado, Beijing Forestry University and the University of Pretoria on the research. The greenhouse study involved a hybrid of two species, Eucalyptus grandis and E. urophylla, that is widely planted in the Southern Hemisphere; there are more than 700 species of eucalyptus, most of them native to Australia.
“Roughly 7% of the world’s forests are plantations, and 25% of that plantation area contains nonnative species and hybrids,” said Elorriaga, now a postdoctoral researcher at North Carolina State. “Eucalyptus is one of the most widely planted genera of forest trees, particularly the 5.7 million hectares of eucalyptus in Brazil, the 4.5 million hectares in China and 3.9 million hectares in India.”